To predict and control the microstructural evolution during dynamic recrystallization, cellular automata (CA) was used to simulate the microstructure evolution of Ti-6Al-4V alloy during hot compressive deformation process. The necessary material constants for CA model was obtained by using the hot compressive flow curves corresponding to the temperatures range from 1000⁰C to 1200⁰C at an interval of 100⁰C and strain rates range from 0.1S^-1 to 10.0S^-1.  Based on the results of hot compressive experiments, the CA model with dynamic recrystallization (DRX-CA) was developed to predict microstructural evolution of dynamic recrystallization during hot compressive deformation of Ti-6Al-4V. The model had the capability of tracking the deformation history of each cell with dislocation density being an internal variable. The simulation results were compared with the experimental results using the hot compressive test. Research results showed that the DRX-CA model established in this paper could accurately reflect the relationship between the flow stress, volume fraction recrystallization and recrystallized grain size and the thermos-mechanical parameters. Simulation results met the flow stress from the experiment. Based on the simulation result, the foundation for later microstructure simulation of Ti-6Al-4V during hot plastic deformation could be laid.